FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (13): 48-51.doi: 10.7506/spkx1002-6630-201013012

• Basic Research • Previous Articles     Next Articles

Separation, Purification and Activity Assay of Antioxidant Peptides from Swimming Crab

FAN Jian-feng,WANG Ze-nan*,YANG Ke,LI Hong-bo,GUO Jun-zhen,ZHOU Xiao-fen   

  1. School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China
  • Received:2009-12-18 Revised:2010-04-19 Online:2010-07-01 Published:2010-12-29
  • Contact: WANG Ze-nan E-mail:wznan@ah163.com

Abstract:

The antioxidant peptides obtained from enzymatic hydrolysis of swimming crab wastes were separated by sequential chromatographic purification with Sephadex G-50 column followed by Sephadex G-25 column. The fractions obtained were evaluated for their scavenging capacities against hydroxyl and superoxide anion free radicals, molecular weight distribution and amino acid composition. The results indicated that subfraction No.3 of fraction No.a of crude antioxidant peptides from swimming crab wastes, the last eluted fraction, more effectively scavenged both of the free radicals and its molecular weight was approximately 1096.5. Totally 11 kinds of amino acids including Tyr, Cys and His with antioxidant activity were obtained through amino acid analysis.

Key words: swimming crab wastes, antioxidant peptides, separation and purification, antioxidant activity, amino acid composition

CLC Number: