Abstract:

Anthocyanins in Morus nigra L. growing in Xinjiang was extracted with ethanol acidified with HCl (95% ethanol plus 0.1% HCl), and determined by pH differential method. The optimum values of extraction parameters including solid-toliquid ratio, length of extraction, extraction temperature and 0.1% HCl-to-95% ethanol ratio were determined by orthogonal array design to be: 1:20, 50 ℃, 90 min and 40:60, respectively, and solid-to-liquid ratio had the most important effect on anthocyanins yield, sequentially followed by length of extraction, extraction temperature and 0.1% HCl-to-95% ethanol ratio. Under the above optimum extraction condions, the anthocyanins yield was 0.0305%. pH differential method presented high reliability in quantifying anthocyanins in Morus nigra L.

Key words: Morus nigra L., anthocyanins, extraction, orthogonal experimental design, pH differential method