Abstract:

This paper reports the use of single factor method and Box-Behnken experimental design combined with response surface methodology for optimizing the water extraction yield of lotus bee pollen polysaccharides (LBPP). In the optimization, a quadratic regression model predicting LBPP yield as a function of 3 extraction conditions, i.e., solid-to-liquid ratio, length of extraction and extraction temperature was set up and the effects of these 3 extraction conditions and their pairwise interactions on LBPP yield were evaluated. All of these extraction conditions had significant effect on LBPP yield and their optimum values were determined as follows: solid-to-liquid ratio 1:9.4 (g/mL) and extraction temperature 81.6 ℃ for an extraction duration of 2.4 h repeated twice. Under such conditions, the predicted and experimental values of LBPP yield were 1.2201% and 1. 2317%, respectively.

Key words: lotus bee pollen polysaccharides, response surface analysis, optimization