FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (14): 205-208.doi: 10.7506/spkx1002-6630-201014046

• Analysis & Detection • Previous Articles     Next Articles

Determination of Total Amount of Sibutramine and Its Active Metabolites in Weight-reducing Food

YANG Chun-hua1，WANG Wei2，LI Gang2,*，LUO Wen-wen3   

  1. 1. Chemical Engineering Department, Jilin Vocational College of Industry and Technology, Jilin 132013, China；
    2. Jilin Institute of Provincial Product Quality Supervision Inspection, Changchun 130022, China ；
    3. School of Chemical Engineering, Changchun University of Technology, Changchun 130012, China
  • Received:2009-09-15 Revised:2010-03-26 Online:2010-07-15 Published:2010-12-29
  • Contact: LI Gang2,*， E-mail:lg56k@yahoo.com.cn

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Abstract:

Objective: To establish a HPLC and HPLC-MS method for determining the total amount of sibutramine and its active metabolites, N-desmethyl sibutramine and N-di-desmethyl sibutramine in weight-reducing food. Methods: Samples were subjected to methylation treatment with formaldehyde and potassium borohydride in ice bath in order to convert active sibutramine metabolites into sibutramine. Results: The linear range of the method was between 10 and 200 mg/L and the regression equation was y = 89.59847x－1.05361 with a correlation coefficient of 0.9999. The detection limit was 1 mg/kg. The average recovery for the total amount of sibutramine and its active metabolites was 83% with a RSD of 4.5%. Conclusion: The established method is simple, accurate and rapid and has good selectivity. It is most suitable for determining total amount of sibutramine and its active metabolites in weight-reducing food.

Key words: sibutramine, active metabolites, HPLC-MS, weight-reducing food

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