FOOD SCIENCE ›› 2019, Vol. 40 ›› Issue (8): 306-310.doi: 10.7506/spkx1002-6630-20171214-178

• Safety Detection • Previous Articles     Next Articles

Simultaneous Determination of 11 Mycotoxins in Minor Food Legumes by High Performance Liquid Chromatography-Tandem Mass Spectrometry (HPLC-MS/MS)

ZHANG Xinna, MA Liyan, PAN Saichao, ZHANG Chunjiao, ZHANG Yongxin, ZHOU Fang, HUANG Kunlun, DAI Yunqing   

  1. 1. Lab of Storage and Preservation, Quality and Safty & Risk Assessment for Agri-Products (Beijing), Ministry of Agriculture, Beijing 100083, China; 2. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China
  • Online:2019-04-25 Published:2019-05-05

Abstract: A method was developed for the simultaneous determination of 11 mycotoxins in minor food legumes, including aflatoxins B1, B2, G1, G2, ochratoxins A, T-2, deoxynivalenol, fumonisins B1, B2 and B3 and zearalenone. Sample extraction and purification conditions were optimized. Samples were extracted with methanol-water (70:30, V/V) and acetonitrilewater (84:16, V/V) consecutively (the extraction process took 3 min), and then the extract was purified using C18 sorbent. The results showed that the calibration curves for all mycotoxins were linear with correlation coefficients above 0.999. The average recoveries of spiked samples ranged from 70.0% to 108%, with relative standard deviations (RSDs) from 1.3% to 8.6%. The method was simple and rapid with high sensitivity and repeatability, and it was suitable for simultaneous detection of 11 mycotoxins in minor food legumes.

Key words: minor food legumes, mycotoxins, high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS)

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