FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (17): 228-231.doi: 10.7506/spkx1002-6630-201017051

• Bioengineering • Previous Articles     Next Articles

Hydrolysis Effect of Heat-resistant Protease on UHT Milk

WANG Hui1,2,LU Jia-ping1,*,LIU Lu1,JIN Lei1
(1. Institute of Agro-food Science and Technology, Key Laboratory of Agricultural Product Processing   

  1. 1. Institute of Agro-food Science and Technology, Key Laboratory of Agricultural Product Processing and Quality Control, Ministry of
    Agriculture, Chinese Academy of Agricultural Sciences, Beijing 100193, China;2. Patent Examination Cooperation Center of State
    Intellectual Property Office, Beijing 100190, China
  • Received:2009-12-18 Online:2010-09-15 Published:2010-12-29
  • Contact: LU Jia-ping E-mail:liquider@sina.com

Abstract:

The effect of heat-resistant protease on protein proteolysis of UHT milk was evaluated by SDS-PAGE and Urea- PAGE. Results indicated that different proteases reacted with different substrates in milk to produce different peptides. Pseudomonas fluorescens protease hydrolyzed κ -casein into para- κ -casein. Plasminogen hydrolyzed α -casein and β -casein into γ-casein, peptone and peptides. In addition, the leftover supernatants after trichloroacetic acid precipitation of the 3 h hydrolysates of UHT milk prepared with plasminogen and bacterial proteases exhibited different RP-HPLC profiles and those of the pH 4.6 soluble peptide in the 3 h hyolroluysates were also different.

Key words: heat-resistant protease, UHT milk, proteolysis

CLC Number: