Abstract:

Objectives: To establish a real-time PCR method for the detection of allergenic almond and peach seeds in food and compare with quantitative test kits for allergenic almond. Methods: On the basis of sequence analysis of the Pru du1 region, specific primers and probes were designed to develop a real-time PCR method using TaqMan probe for the identification of allergenic almond or peach seed residues in food. In order to compare with the test kits, recovery rates and detection limit of established real-time PCR were determined using the reference material of allergenic almond. Results: The established real-time PCR method was highly specific for the detection of allergenic almond. Its sensitivity was less than 10 mg/kg. Conclusion: The real-time PCR method is highly sensitive and specific, thereby providing an applicable method for the detection of allergenic almond and peach seeds in food.

Key words: almond, allergenic Pru du1 gene, fluorescence quantitative polymerase chain reaction