Visible Spectrophotometric Determination of Total Flavonoids in Capparis spinosa L. Buds

doi:10.7506/spkx1002-6630-201018058

FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (18): 252-254.doi: 10.7506/spkx1002-6630-201018058

• Analysis & Detection • Previous Articles Next Articles

Visible Spectrophotometric Determination of Total Flavonoids in Capparis spinosa L. Buds

JIANG Xiu-juan1,2，TANG Jin-cheng2，BAI Hong-jin1,2,*

1. Key Laboratory of Protection and Utilization of Biological Resource in Tarim Basin of Xinjiang Production and Construction Corps,
Alar 843300, China；2. College of Life Science, Tarim University, Alar 843300, C

Received:2009-11-05 Revised:2010-05-21 Online:2010-09-25 Published:2010-12-29
Contact: JIANG Xiu-juan1,2， E-mail:jxjuan2010@126.com

Abstract

Abstract:

Different extraction methods were coupled with visible spectrophotometry to develop a simple and accurate method for quantifying total flavonoids in Capparis spinosa L. buds. The detection wavelength was set at 500 nm. Rutin was used as the standard sample and NaNO3, Al(NO3)3 and NaOH was used to compose the coloration system. Results indicated that the methanol reflux method exhibited the highest extraction rate for total flavonoids. A good linear relationship was observed in the range of 0 to 32 mg/L (r = 0.9997) and average recovery rate was 100.3% with a relative standard deviation of 0.98% (n=6). The average content of total flavonoids in the samples from Kuche county, Xinjiang autonomous region was 1.13%.

Key words: Capparis spinosa L. buds, total flavonoids, spectrophotometry, determination

CLC Number:

O657.32

JIANG Xiu-juan1,2，TANG Jin-cheng2，BAI Hong-jin1,2,*. Visible Spectrophotometric Determination of Total Flavonoids in Capparis spinosa L. Buds[J]. FOOD SCIENCE, 2010, 31(18): 252-254.

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Visible Spectrophotometric Determination of Total Flavonoids in Capparis spinosa L. Buds

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