FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (20): 52-57.doi: 10.7506/spkx1002-6630-201020011

• Processing Technology • Previous Articles     Next Articles

Effect of Proteolytic Pre-treatment on the Extraction of Xanthophylls from Corn Gluten Meal

ZHOU Hai-sheng,JIANG Ling-zhi,HUANG Mei*   

  1. Department of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China
  • Received:2010-01-08 Revised:2010-06-12 Online:2010-10-25 Published:2010-12-29
  • Contact: HUANG Mei E-mail:huangm@zju.edu.cn

Abstract:

After being pre-treated with protease, corn gluten meal received organic solvent extraction to obtain xanthophylls. Neutral protease was selected out of three commercially available proteases to pretreat corn gluten meal due to the highest extraction efficiency of xanthophylls, and methanol was found to be the best solvent for the extraction of xanthophylls among the ten solvents tested. The optimal conditions for neutral protease hydrolysis were determined to be: enzyme load, 14000 U/g substrate, liquid/solid ratio, 20:1; reaction temperature 40 ℃; and reaction duration, 5 h. Compared with direct extraction without proteolytic pre-treatment, the optimized neutral protease hydrolysis resulted in an increase of extraction efficiency of xanthophylls from 165.3 to 340.5 μg/g and a reduction of extraction duration from 5 hour to 20 min.

Key words: xanthophylls, corn gluten meal, proteolytic hydrolysis, extraction

CLC Number: