Screening and Identification of a Pseudomonas lutea Strain Capable of Degrading Caffeine

doi:10.7506/spkx1002-6630-201021049

FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (21): 218-221.doi: 10.7506/spkx1002-6630-201021049

• Bioengineering • Previous Articles Next Articles

Screening and Identification of a Pseudomonas lutea Strain Capable of Degrading Caffeine

LI Na1，JI Bao-ping1,*，LI Bo1，WU Wei2

1. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China；
2. College of Engineering, China Agricultural University, Beijing 100083,China

Received:2010-04-20 Online:2010-11-15 Published:2010-12-29
Contact: JI Bao-ping E-mail:jbp@cau.edu.cn

Abstract

Abstract:

Objective: The aim of this work was to find strains capable of degrading caffeine so as to develop a biological approach for caffeine detoxicification. Method: The preliminary screening of target strains was performed using caffeine as both carbon and nitrogen sources, and then the ten screened strains were further screened by evaluating their abilities to degrade caffeine in liquid medium after culture. As a result, a strain with the strongest ability to degrade caffeine was obtained and named HZ-1. After 48 h culture of HZ-1, caffeine in liquid medium was completely degraded. Based on the morphological, physiological and biochemical properties and 16 rDNA gene sequence, this strain was identified as Pseudomonas lutea. To the best of our knowledge, this is the first report on the biological degradation of caffeine at home.

Key words: caffeine, Pseudomonas lutea, screening, identification

CLC Number:

TS201.3

LI Na1，JI Bao-ping1,*，LI Bo1，WU Wei2. Screening and Identification of a Pseudomonas lutea Strain Capable of Degrading Caffeine[J]. FOOD SCIENCE, 2010, 31(21): 218-221.

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Screening and Identification of a Pseudomonas lutea Strain Capable of Degrading Caffeine

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