Enzymatic Preparation of Angiotensin Ⅰ -converting Enzyme (ACE) Inhibitory Products Derived from Viscera of Channel Catfish

doi:10.7506/spkx1002-6630-201022050

FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (22): 237-241.doi: 10.7506/spkx1002-6630-201022050

• Processing Technology • Previous Articles Next Articles

Enzymatic Preparation of Angiotensin Ⅰ -converting Enzyme (ACE) Inhibitory Products Derived from Viscera of Channel Catfish

YANG Xiao-jun，LU Jian-feng*，LIN Lin，DUAN Rui-xia，WENG Shi-bing，YE Ying-wang，JIANG Shao-tong

School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China

Received:2010-06-30 Revised:2010-11-09 Online:2010-11-25 Published:2010-12-29
Contact: LU Jian-feng E-mail:lujf@sibs.ac.cn

Abstract

Abstract:

In this study, viscera of channel catfish, Ictalurus punctatus, were used as the raw materials to prepare angiotensin Ⅰ-converting enzyme (ACE) inhibitory products on the basis of its inhibitory rate of ACE. Papain was identified as the optimal enzyme. The effects of enzymatic hydrolysis time, enzyme addition amount, initial pH, hydrolysis temperature, and liquidmaterial ratio on the inhibitory rate of prepared ACE inhibitory products were investigated by orthogonal experiments. Results indicated that the optimal enzymatic hydrolysis conditions were initial pH of 7.5, hydrolysis temperature of 60 ℃, and liquidmaterial ratio of 2:1. Under the optimal conditions, the ACE inhibitory rate of enzymatic hydrolysate reached up to 72.34%.

Key words: Ictalurus punctatus, fish viscera, inhibitory rate, angiotensin Ⅰ -converting enzyme, papain

CLC Number:

TS254.9

YANG Xiao-jun，LU Jian-feng*，LIN Lin，DUAN Rui-xia，WENG Shi-bing，YE Ying-wang，JIANG Shao-tong. Enzymatic Preparation of Angiotensin Ⅰ -converting Enzyme (ACE) Inhibitory Products Derived from Viscera of Channel Catfish[J]. FOOD SCIENCE, 2010, 31(22): 237-241.

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Enzymatic Preparation of Angiotensin Ⅰ -converting Enzyme (ACE) Inhibitory Products Derived from Viscera of Channel Catfish

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