FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (22): 394-397.doi: 10.7506/spkx1002-6630-201022087

• Analysis & Detection • Previous Articles     Next Articles

HPLC-MS/MS Determination of Nandrolone and Nandrolone Phenylpropionate Residues in Aquatic Products

HUANG Dong-mei,SHI Yong-fu,WANG Yuan,CAI You-qiong   

  1. (East China Sea Fishery Research Insititute, Chinese Academy of Fishery Sciences, Shanghai 200090, China)
  • Received:2010-07-04 Online:2010-11-25 Published:2010-12-29
  • Contact: CAI You-qiong E-mail:caiyouqiong@163.com

Abstract:

A method was established for the determination of nandrolone and nandrolone phenylpropionate residues in aquatic products. Samples were extracted with mixed solution after enzymatic hydrolysis, purified with liquid-liquid extraction. Then the impurities were removed by C18 column, and the eluant was concentrated and subjected to liquid chromatography-tamden mass spectrometry analysis. Good linear correlation was obtained between the peak area and the concentration in the range of 0.005 0.25μg/mL. The spike recoveries were 76.8%-98.6% and 84.2%-91.3% for nandrolone and nandrolone phenylpropionate standard. The relative standard deviations were all below 13.6%, and the limits of detection was 5.0μg/kg.

Key words: liquid chromatography-tandem mass spectrometry, nandrolone phenylpropionate, residue, aquatic products

CLC Number: