Purification and Identification of Donkey s Milk Protein Fractions

doi:10.7506/spkx1002-6630-201023011

FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (23): 44-48.doi: 10.7506/spkx1002-6630-201023011

• Basic Research • Previous Articles Next Articles

Purification and Identification of Donkey s Milk Protein Fractions

SU Wei1，YANG Jie2,*，SHEN Xiao-li3，JIANG Xin-yue3Wei Su

College of Life Science and Technology, Xinjiang University, Urumqi 830046,China

Received:2010-09-28 Revised:2010-11-12 Online:2010-12-15 Published:2010-12-29
Contact: YANG Jie E-mail:xindayangjie@sina.com

Abstract

Abstract:

Objective: To provide a theoretical basis for further exploring the exploitability of donkey milk as an adjuvant for disease treatment or as a substitute for human milk. Methods: The protein fractions of donkey whey were separated and purified by DEAE-52 anion-exchange chromatography and Sephadex G-100 gel-filtration chromatography. The purified protein fractions were identified by SDS-PAGE and high-performance gel permeation chromatography. Results: Three unknown proteins having molecular masses of 32, 70.1 kD and 72.2 kD were found to be present in donkey whey in comparison with the protein composition profile of cow s milk whey. Conclusion: Along with the similar nutritional components to those in cow s milk, other bioactive components were also contained in donkey s milk, which might be some protective proteins playing an important role in protecting the organism against diseases.

Key words: donkey s milk, protein fraction, separation, purification

CLC Number:

TS252

SU Wei1，YANG Jie2,*，SHEN Xiao-li3，JIANG Xin-yue3. Purification and Identification of Donkey s Milk Protein Fractions[J]. FOOD SCIENCE, 2010, 31(23): 44-48.

[1]	XIANG Ting, XIA Chen, LIU Jianhua, WANG Chaoran, SHEN Jianfu. Separation, Structural Identification and Anti-tumor Effects of New Compounds from Cordyceps militaris [J]. FOOD SCIENCE, 2021, 42(8): 235-242.
[2]	WANG Wei, LI Jinjin, CHI Hai. Purification and Antimicrobial Mechanism of Amylocyclicin W5 Produced by Bacillus amyloliquefaciens DH8030 [J]. FOOD SCIENCE, 2021, 42(7): 29-34.
[3]	WEI Chenye, BAO Xiaowei, WANG Juan, HE Mengmeng, ZENG Lanjun, ZHANG Yatao. Isolation, Purification and Antioxidant Activity of Polysaccharides from the Fruit of Hippophae rhamnoides [J]. FOOD SCIENCE, 2021, 42(4): 227-232.
[4]	HU Xiao, ZHOU Ya, CHEN Xingxing, LI Laihao, YANG Xianqing, CHEN Shengjun, WU Yanyan, YANG Shaoling. Preparation, Purification and in Vitro Evaluation of Antioxidant Peptides from Tilapia (Oreochromis niloticus Sarcoplasmic Protein [J]. FOOD SCIENCE, 2021, 42(3): 63-70.
[5]	YANG Qile, DING Yifeng, ZHANG Yu, NIE Ruonan, LI Yameng, WANG Jia, WANG Xiaohong. Sequence Analysis and Binding Activity of Salmonella Phage LPST144 Tail Fiber gp38 [J]. FOOD SCIENCE, 2021, 42(2): 66-73.
[6]	GAO Zhaojian, WANG Qiufen, DING Feihong, XU Xiang, ZHAO Yifeng, JIAO Wei, CHEN Teng. Screening and Mutagenesis of Broad-Spectrum Antagonistic Bacillus licheniformis and Purification and Identification of Antimicrobial Substances Produced by Its Mutant [J]. FOOD SCIENCE, 2021, 42(2): 143-150.
[7]	LIU Lu, WU Jiangli, YANG Jintao, TANG Zhongyue, ZENG Xuefeng. Isolation and Fermentation Characteristics of γ-Aminobutyric Acid-producing Lactic Acid Bacteria from Yujiangsuan, a Traditional Miao Ethnic Fermented Condiment [J]. FOOD SCIENCE, 2021, 42(18): 73-79.
[8]	JIANG Yujun, YANG Tao, MAN Chaoxin, ZHOU Wenqi, WANG Rui, CHEN Sihan, YANG Xinyan, FU Shiqian, GAO Pingping, REN Yuwei, ZHANG Wei. Rapid Detection of Cronobacter sakazakii in Powdered Infant Formula by Immunomagnetic Separation Coupled with Visual Nanogold Hybridization Probe [J]. FOOD SCIENCE, 2021, 42(16): 224-232.
[9]	LI Yujing, SUN Liping, ZHUANG Yongliang. Purification and Thermal Stability of Geraniin from Rambutan (Nephelium lappaceum) Peels [J]. FOOD SCIENCE, 2021, 42(15): 44-49.
[10]	GAO Zhaojian, DING Feihong, CHEN Huan, GENG Yunlong, ZHAO Yifeng. Purification and Characterization of Antifungal Chitinase from Penicillium chrysogenum [J]. FOOD SCIENCE, 2021, 42(14): 129-136.
[11]	HUANG Qinqin, FAN Fengjiao, LI Peng, FAN Yan, ZOU Yanyu, FANG Yong. Analysis of Alkylresorcinol Homologues in Wheat Flour by GC-MS and LC-MS/MS [J]. FOOD SCIENCE, 2021, 42(14): 187-194.
[12]	ZHANG Biao, HUANG Na, ZHANG Wanli, WANG Shuo, SHENG Wei. Fluorescence Immunoassay Combined with Magnetic Separation Using Upconversion Nanoparticles as Label for the Detection of Tyramine in Food Samples [J]. FOOD SCIENCE, 2021, 42(14): 270-275.
[13]	YANG Ruqing, CHEN Shoufeng, XIAO Linlin, CHEN Yulei, SUN Lechang, ZHANG Lingjing, LIU Guangming, CAO Minjie. Prolyl Endopeptidase from Sea Bass (Lateolabrax japonicus): Purification, Characterization and Molecular Cloning [J]. FOOD SCIENCE, 2021, 42(14): 78-85.
[14]	SHA Yuhuan, MAO Xiaoying, WU Qingzhi, ZHANG Jian, CHENG Weidong. Flavonoid Composition and Antioxidant Activity of Diaphragma juglandis Fructus [J]. FOOD SCIENCE, 2021, 42(12): 91-98.
[15]	CUI Xiaoying, PENG Xinyan, HE Hongjun, ZHANG Min, ZHANG Xiaotong. Separation, Purification and Enzymatic Characterization of Polyphenol Oxidase from Taro [J]. FOOD SCIENCE, 2021, 42(12): 107-115.

Purification and Identification of Donkey s Milk Protein Fractions

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments