FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (24 ): 230-235.doi: 10.7506/spkx1002-6630-201024049

• Processing Technology • Previous Articles     Next Articles

Optimization of Extraction Processing for Water-soluble Polysaccharides from Flammulina velutipes

KONG Xiao-xue,AN Xin-xin*,ZHAO Li-yan,HU Qiu-hui   

  1. College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China
  • Received:2010-11-02 Online:2010-12-25 Published:2010-12-29
  • Contact: AN Xin-xin E-mail:anxinxin@njau.edu.cn

Abstract:

Wet ultra-fine pulverization extraction, microwave-assisted extraction and ultrasound-assisted extraction were used to explore the optimal extraction conditions of water-soluble polysaccharides from Flammulina velutipes. Response surface methodology (RSM) was employed to optimize the conditions of wet ultra-fine pulverization extraction for water-soluble polysaccharides. Results indicated that the optimal extraction processing parameters were the gap of colloid mill at 42μm, extraction temperature of 87 ℃ and extraction time of 170 min. Under the optimal extraction conditions, the extraction rate of polysaccharides was 3.63%. The concentration of water-soluble polysaccharides based on predictive model was 417.6μg/mL, and the actually determined concentration of water-soluble polysaccharides was (418 ± 3.21 )μg/mL.

Key words: Flammulina velutipes, water-soluble polysaccharides, wet ultra-fine pulverization extraction, response surface methodology (RSM)

CLC Number: