Determination of Ampicillin Residues in Milk by Receptor-binding Assay

doi:10.7506/spkx1002-6630-201024054

FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (24): 255-258.doi: 10.7506/spkx1002-6630-201024054

• Analysis & Detection • Previous Articles Next Articles

Determination of Ampicillin Residues in Milk by Receptor-binding Assay

SUN Yong-hai1，ZHANG Jie2，HAN Yong3，LI Zhuo-lin2，LI Tie-zhu2,*

1. College of Biological and Agricultural Engineering, Jilin University, Changchun 130025, China；
2. Center of Agro-Food Technology, Jilin Academy of Agricultural Sciences, Changchun 130124, China；
3. China International Engineering Consulting Corporation, Beijing 100048, China

Received:2010-01-13 Online:2010-12-25 Published:2010-12-29
Contact: LI Tie-zhu E-mail:ltzjlu@126.com

Abstract

Abstract:

The receptor has been utilized to develop a novel microplate assay for the determination of ampicillin with intact beta-lactam structure in milk. The receptor with high affinity to penicillin is produced by biotechnology. Then the receptor is employed as an identification unit to develop an indirect competitive receptor-binding assay. The detection limit of ampicillin was 2 μg/kg in milk. The assay has been developed as screening test with the option for a quantitative assay, when the identity of the residual beta-lactam is known.

Key words: milk, ampicillin, receptor binding assays, biotin, avidin

CLC Number:

S859. 84

SUN Yong-hai1，ZHANG Jie2，HAN Yong3，LI Zhuo-lin2，LI Tie-zhu2,*. Determination of Ampicillin Residues in Milk by Receptor-binding Assay[J]. FOOD SCIENCE, 2010, 31(24): 255-258 https://doi.org/10.7506/spkx1002-6630-201024054

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Determination of Ampicillin Residues in Milk by Receptor-binding Assay

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