FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (24): 264-267.doi: 10.7506/spkx1002-6630-201024056

• Analysis & Detection • Previous Articles     Next Articles

AFLP Fingerprinting for Identifying Geographical Origins of 31 Sun-dried Tea (Maocha) Samples

JI Peng-zhang1,2，LU Cai-you3，LIANG Ming-zhi1，ZHANG Jun1，HUANG Xing-qi2   

  1. 1. Institute of Tea Research, Yunnan Academy of Agricultural Sciences, Kunming 650203, China；
    2. Institute of Biotechnology and Genetic Germplasm, Yunnan Academy of Agricultural Sciences, Kunming 650203, China；
    3. College of Pu er Tea, Yunnan Agricultural University, Kunming 650201, China)
  • Received:2010-08-19 Revised:2010-11-15 Online:2010-12-25 Published:2010-12-29
  • Contact: JI Peng-zhang E-mail:pengzhji@126.com

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Abstract:

Amplified fragment length polymorphisms (AFLPs) markers were employed to identify the geographical origins of 31 sundried tea samples, which was a material for Pu erh tea processing. A total of 420 polymorphic bands out of 426 reproducible products were amplified. Percentage of polymorphic loci (P) was 94.6%. Dendrogram generated after UPGMA clustering by NTSYS software indicated that 31 samples could be divided into conventional and ancient tea type. There were three methods to identify ancient tea and conventional tea, and to discriminate ancient tea from other geographical origins using AFLP marker: (1) based on the main peak value and the highest and second highest value of dye signal of tea samples. (2) based on the whole AFLP diagram of different samples. (3) cmpare target tea AFLP diagram with control tea group AFLP diagram. The result showed that AFLP marker was an effective tool and a robust method in discrimination of sun-dried tea (Maocha).

Key words: Pu erh tea, sun-dried tea, amplified fragment length polymorphism (AFLP)

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