FOOD SCIENCE ›› 2011, Vol. 32 ›› Issue (1 ): 14-18.doi: 10.7506/spkx1002-6630-201101004

• Basic Research • Previous Articles     Next Articles

Comparative Study on Bacteriostasis of Different Preservatives for Three Typical Spoilage Bacteria

CHEN Nan-nan1,XU Xin1,SHANG Feng-cai1,HUANG Qin1,CHEN You-liang2,LI Wei-fen1,*   

  1. 1. Key Laboratory of Molecular Animal Nutrition, Ministry of Education, Institute of Feed Science, College of Animal Science,
    Zhejiang University, Hangzhou 310029, China;2. Laboratory of Animal Product Processing, College of Animal Science,
    Zhejiang University, Hangzhou 310029, China
  • Received:2010-03-22 Revised:2010-12-07 Online:2011-01-15 Published:2010-12-28
  • Contact: LI Wei-fen E-mail:wfli@zju.edu.cn

Abstract:

Objective: The inhibitory effects of twenty-three food preservatives on three typical spoilage bacteria were evaluated and compared. Methods: The minimal inhibitory concentrations (MIC) of different preservatives for Micrococcus luteus, Bacillus subtilis and E.coli were determined by micro-dilution assay. Results: Among the 23 tested food preservatives, Nisin and ε-poly-L-lysine had the best inhibitory effect on Micrococcus luteus (G+ cocci), and their MICs were both 12.5 μg/mL. For Bacillus subtilis (G+ bacillus), the best antimicrobials were calcium propionate, diacetyl, citric acid, citral and D-sodium isoascorbiate, presenting an identical MIC of 6.25μg/mL. As for E.coli (G- bacillus), hydrogen peroxide and diacetyl were the best preservatives, with an identical MIC of 25μg/mL. Conclusion: The bacteriostasis of various tested preservatives for the three typical spoilage bacteria is irregular and their combined use seems to be an approach for more effective bacteriostasis.

Key words: preservative, spoilage bacteria, minimal inhibition concentration, bacteriostasis

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