Abstract:

A crude globulin extract from naked oat seeds was prepared by Osborne method and purified by salting-out and Sephadex G-100 gel filtration chromatography, and the crude extract was separated into two fractions (I and II). The crude globulin extract, its salting-out purification product and fractions I and II were subjected to SDS-PAGE analysis and antioxidant activity evaluation. Oat seed globulins exhibited 60% degree of saturation with ammonium sulphate. The molecular weight distribution of fraction I consisted of 44, 34 － 29kD and25 kD, and that of fraction I was 25, 23, 19 kD. Among the four materials, fraction I had the strongest scavenging effect on hydroxyl, superoxide anion and DPPH free radicals, with IC50 values of 3.73, 0.016 mg/mL and 1.033 mg/mL, respectively, and its hydroxyl free radical scavenging effect was higher than that of VC. However, the free radical scavenging effect of fraction II was lower than those of all three other materials.

Key words: naked oat, globulin, purification, antioxidant