Optimization of Culture Medium and Liquid-state Fermentation Conditions for Polygalacturonase Production by Thermophilic sporotrichum

doi:10.7506/spkx1002-6630-201101028

FOOD SCIENCE ›› 2011, Vol. 32 ›› Issue (1 ): 115-119.doi: 10.7506/spkx1002-6630-201101028

• Bioengineering • Previous Articles Next Articles

Optimization of Culture Medium and Liquid-state Fermentation Conditions for Polygalacturonase Production by Thermophilic sporotrichum

ZHANG Hui，SANG Qing，LIU Jin-sen，SUN Zhan-bin

(School of Life Sciences, Liaocheng University, Liaocheng 252059, China )

Received:2010-01-29 Revised:2010-12-06 Online:2011-01-15 Published:2010-12-28
Contact: ZHANG Hui E-mail:zhanghui2005@lcu.edu.cn

Abstract

Abstract:

Thermophilic sporotrichum was cultured in a liquid medium with shaking for polygalacturonase production in this study. To improve polygalacturonase production, the culture medium composition and four fermentation conditions were optimized. The optimal culture medium composition found was composed of wheat bran 1.5%, (NH4)2SO4 0.5%, apple peel powder 3%, KH2PO4 0.1% and MgSO4·7H2O 0.025%, pH 6.0. The optimal values of the volume of the medium in a 250 mL conical flask, fermentation temperature and duration and shaking speed were determined to be 45 mL, 50 ℃, 96 h and 170 r/min, respectively. The fermentation broth obtained under the optimized conditions presented a polygalacturonase activity of 2768.97 U/mL.

Key words: polygalacturonase, Thermophilic sporotrichum, liquid-state fermentation

CLC Number:

TQ925

ZHANG Hui，SANG Qing，LIU Jin-sen，SUN Zhan-bin. Optimization of Culture Medium and Liquid-state Fermentation Conditions for Polygalacturonase Production by Thermophilic sporotrichum[J]. FOOD SCIENCE, 2011, 32(1 ): 115-119.

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Optimization of Culture Medium and Liquid-state Fermentation Conditions for Polygalacturonase Production by Thermophilic sporotrichum

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