Abstract:

Objective: To investigate the effect of resveratrol on insulin sensitivity in insulin-resistant KKAy mice and to probe the mechanism. Methods: A total of 36 7-week-old KKAy mice were randomly divided into 3 groups, which were fed standard AIN93G diet or standard AIN93G diet with resveratrol at contents of 2 g/kg and 4 g/kg diet. Totally 12 C57BL/6J mice were used as the control. The 12 h fasting levels of blood glucose and serum insulin and glucose and insulin tolerance at 0, 6 weeks and 12 weeks of drug intervention were determined to evaluate the effect of resveratrol. The expression of silent information regulator(sirt-1) and phosphorylation of insulin receptor substrate 1 (IRS-1) in liver were examined by western blotting. Results: Resveratrol significantly improved insulin sensitivity of KKAy mice, ameliorated glucose tolerance and insulin tolerance, and enhanced the expression level of sirt-1 and phosphorylation of IRS-1 in liver. Conclusion: Resveratol may ameliorate insulin resistance and glucose tolerance disorder of mice due to its promotion effect on the expression of sirt-1 in liver and activation effect on the proteins related to insulin signaling.

Key words: resveratrol, insulin resistance, silent information regulator-1, insulin receptor substrate-1