Separation and Purification of Aucubin from Eucommia ulmoides Seed Meal

doi:10.7506/spkx1002-6630-201102008

FOOD SCIENCE ›› 2011, Vol. 32 ›› Issue (2 ): 32-35.doi: 10.7506/spkx1002-6630-201102008

• Processing Technology • Previous Articles Next Articles

Separation and Purification of Aucubin from Eucommia ulmoides Seed Meal

LI Hui1,2，WEN Chi-fu1,2，LI Ya-nan1，PENG Yu-dan1，WANG Lan1，ZHANG Yong-kang1,2

1. College of Biology and Environmental Science, Jishou University, Jishou 416000, China；
2. Hunan Provincial Key Laboratory of Plant Resource Protection and Exploitation, Jishou University, Jishou 416000, China

Received:2010-04-02 Revised:2010-12-01 Online:2011-01-25 Published:2011-01-10
Contact: LI Hui E-mail:lihuijsdx@163.com

Abstract

Abstract:

The objective of this study was to develop a macroporous resin adsorption method for separating and purifying aucubin in Eucommia ulmoides seed meal extracted into 80% aqueous ethanol solution. Twelve kinds of macroporous resins were compared for differences in their adsorption and desorption performances towards aucubin, and the results indicated that macroporous resins SIPI-7, HZ-820 and S-8 had better abilities to adsorb and desorb aucubin, among which, HZ-820 was considered to be the best resin due to the highest desorption rate. The optimal conditions for the adsorption and desorption of HZ-820 were found to be: sample loading, 0.5 g/g (weight ratio of Eucommia ulmoides seed meal extracted to HZ-820 resin); desorpion solvent, 50% aqueous ethanol solution containing 3% acetic acid at a flow rate of 1.0 mL/min. The high aucubin eluate fractions obtained under such conditions were pooled, condensed and recrystallized into a considerable amount of product containing 96.82% of aucubin.

Key words: aucubin, separation and purification, macroporous resin, Eucommia ulmoides seeds

CLC Number:

TS201.1

LI Hui1,2，WEN Chi-fu1,2，LI Ya-nan1，PENG Yu-dan1，WANG Lan1，ZHANG Yong-kang1,2. Separation and Purification of Aucubin from Eucommia ulmoides Seed Meal[J]. FOOD SCIENCE, 2011, 32(2 ): 32-35.

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Separation and Purification of Aucubin from Eucommia ulmoides Seed Meal

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