Abstract:

The residue of Rana amurensis Boulenger after oil extraction was hydrolyzed by alkaline protease to extract collagen into the supernatant. The effects of initial hydrolysis pH, enzyme loading, and hydrolysis temperature and time on collagen yield were investigated by single factor experiments and response surface methodology. The optimum values of the four conditions for improved collagen extraction were determined as follows: initial hydrolysis pH 10; enzyme loading 1.5%; hydrolysis temperature 50 ℃; and hydrolysis time 2.5 h. Under the optimized conditions, a collagen yield of 55.38% was attained, with a product purity of 64.15%.

Key words: alkaline protease, Rana amurensis Boulenger residue, collagen, response surface methodology