FOOD SCIENCE ›› 2011, Vol. 32 ›› Issue (2 ): 157-163.doi: 10.7506/spkx1002-6630-201102037
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IU Dao-liang，ZHAO Zhan-min，HU Lian-xia，ZHANG Jun-feng，SUN Xiao-xia
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Abstract: The total numbers of bacteria in liquid foods such as milk and fruit juice were determined by a flow cytometry (FCM) method. A detection limit of 10 CFU/mL was achieved by removing the non-microorganism particles in fruit juice through membrane filtration and centrifugation. The reproducibility was tested to verify the reliability of the method in determining milks by comparing with the standard plate count (SPC) method. The FCM result was linearly correlated with that of SPC within a certain concentration range. FCM is faster and more accurate than SPC methods in determining the total number of bacteria in liquid samples.
Key words: flow cytometry method, total bacterial number, liquid foods
CLC Number:
TS201.3
IU Dao-liang，ZHAO Zhan-min，HU Lian-xia，ZHANG Jun-feng，SUN Xiao-xia. Rapid Determination of Total Bacterial Number in Liquid Foods by A Flow Cytometry Method[J]. FOOD SCIENCE, 2011, 32(2 ): 157-163.
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URL: https://www.spkx.net.cn/EN/10.7506/spkx1002-6630-201102037
https://www.spkx.net.cn/EN/Y2011/V32/I2 /157
