FOOD SCIENCE ›› 2011, Vol. 32 ›› Issue (2 ): 187-190.doi: 10.7506/spkx1002-6630-201102044

• Analysis & Detection • Previous Articles     Next Articles

Simultaneous Determination of Six Carbamate Pesticide Residues in Milk by HPLC-ESI-MS/MS

TIAN Hong-zhe,ZHAO Ying-bo,ZHOU Yan-ming   

  1. Analysis and Testing Center, Shenyang Agricultrual University, Shenyang      110866, China
  • Received:2010-03-08 Revised:2010-11-29 Online:2011-01-25 Published:2011-01-10
  • Contact: TIAN Hong-zhe E-mail:tthz2@yahoo.com.cn

Abstract: A high performance liquid chromatography-ion trap mass spectrometric (HPLC-ESI-MS/MS) method was developed to determine six carbamate pesticide residues in milk samples simultaneously. Samples were extracted with acetonitrile, cleaned up on an Oasis HLB-SPE column, separated on a C18 column using mixed methanol and 0.02 mol/L ammonium acetate as a mobile phase (60:40, V/V) by gradient elution, followed by multiple reaction monitoring (MRM) mode detection. The limits of quantification ranged from 1.0 to 10.0μg/kg. The average recoveries in spiked samples were between 79.21% and 91.38% with relative standard deviations from 3.44% to 5.71%. The proposed method can meet the current requirements for the analysis residual pesticides.

Key words: high performance liquid chromatography-electrospray ionization mass spectrometry, insectcide, carbamate pesticide residue, milk

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