Abstract: A high performance liquid chromatography (HPLC) method was used to determine the contents three soluble sugars (fructose, sucrose and glucose) in fresh corn. The chromatographic column used was an Agilent Zorbax carbohydrate (4.6 mm × 250 mm, 5 μm) column held at 25 ℃, and the mobile phase was a mixture of acetonitrile and water (V/V, 75:25) at a flow rate of 1 mL/min. The established cabliration curves of fructose, sucrose and glucose displayed correlation coefficients ranging from 0.99839 to 0.99995 (n = 5). In precision experiments, relative standard deviations (RSDs) between 0.90% and 3.8% were obtained (n = 5). The average spike recoveries of the method ranged from 95.28% to 101.02%. This method can be used for accurate, reasonable and rapid analysis of soluble sugar in fresh corn.

Key words: fresh corn, soluble sugar, high performance liquid chromatography (HPLC)