Sequence and Classification Analysis of 18S rDNA in Asparagus officinalis L.

doi:10.7506/spkx1002-6630-201105047

FOOD SCIENCE ›› 2011, Vol. 32 ›› Issue (5 ): 221-223.doi: 10.7506/spkx1002-6630-201105047

• Bioengineering • Previous Articles Next Articles

Sequence and Classification Analysis of 18S rDNA in Asparagus officinalis L.

HOU Jin-hui，CAI Kan，ZHENG Bao-gang，CAO Yu-peng，WANG Qing-quan

College of Food (Biological) Engineering, Xuzhou Institute of Technology, Xuzhou 221008, China

Received:2010-05-31 Revised:2011-02-09 Online:2011-03-15 Published:2011-03-03
Contact: HOU Jin-hui E-mail:houjinhui0@126.com

Abstract

Abstract: In this study, the genomic DNA of Asparagus officinalis L. was extracted by plant genome extraction method, and its fragment of 18S rDNA was amplified by PCR method, followed by ligation, transformation, and screening. An 18S rDNA fragment of 1684 bp in length was sequenced. The accession number of the sequence in Genbank was GU217543. The gene sequence of Asparagus officinalis L. was analyzed. The results indicated that Asparagus officinalis L. had close genetic relationship with the species of Liliales and Asparagales. Based on these investigations, the classification of Asparagus officinalis L. has been further understood at the molecular level.

Key words: Asparagus officinalis L., 18S rDNA, classification

CLC Number:

S59.023

HOU Jin-hui，CAI Kan，ZHENG Bao-gang，CAO Yu-peng，WANG Qing-quan. Sequence and Classification Analysis of 18S rDNA in Asparagus officinalis L.[J]. FOOD SCIENCE, 2011, 32(5 ): 221-223.

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Sequence and Classification Analysis of 18S rDNA in Asparagus officinalis L.

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