Abstract: A reverse phase high performance liquid chromatography (RP-HPLC) method has been developed for determining dinotefuran, clothianidin, imidacloprid and acetamiprid residues in rice. Neonicotinoid residues were extracted from the sample by acetonitrile, and then cleaned up with ENVI-Florisil and carb SPE tube. The HPLC detection was performed using a DAD detector in the external standard mode. Results indicated that average recovery rates in spiked samples were in the range of 75.5%－96.0%, the relative standard deviations varied between 0.49% and 4.36% and the detection limit was 0.004 mg/kg. A good linear correlation between peak areas and the concentrations of four neonicotinoid residues was observed in the range of 0.1－1000μg/mL with a calibration coefficient of 0.9996.

Key words: dinotefuran, clothianidin, imidacloprid, acetamiprid, high performance liquid chromatography, solid phase extraction, rice