Determination of SOD Specific Activity in Animal and Plant Tissues by Improved Xanthine Oxidase Method

doi:10.7506/spkx1002-6630-201106055

FOOD SCIENCE ›› 2011, Vol. 32 ›› Issue (6 ): 237-241.doi: 10.7506/spkx1002-6630-201106055

• Analysis & Detection • Previous Articles Next Articles

Determination of SOD Specific Activity in Animal and Plant Tissues by Improved Xanthine Oxidase Method

XU Dong，ZHAO Jian，HUANG Han-chang，WEN Jing*

Beijing Key Laboratory of Bioactive Substances and Functional Foods, Beijing Union University, Beijing 100191, Ch

Received:2010-04-20 Revised:2011-02-13 Online:2011-03-25 Published:2011-03-03
Contact: WEN Jing* E-mail:wenjing@ygi.edu.cn

Abstract

Abstract: In order to minimize the interference in xanthine oxidase method for the determination of superoxide dismutase (SOD) activity in animal and plant tissues, the xanthine oxidase method was modified. The optimal experimental conditions for preparing bank samples were explored by measuring the activity and circular dichroism spectrum of SOD. A heated sample was designed as the experimental blank. Subtraction rule was used to eliminate the interference of other substances in xanthine oxidase system. Standard addition recovery experiments and actual determination of SOD activity in animal and plant tissues were used to compare the method with and without modification. Results indicated that the improved xanthine oxidase method could result in more accurate determination of SOD activity in animal and plant tissues.

Key words: xanthine oxidase method, sample blank, SOD activity determination

CLC Number:

TS207.3

XU Dong，ZHAO Jian，HUANG Han-chang，WEN Jing*. Determination of SOD Specific Activity in Animal and Plant Tissues by Improved Xanthine Oxidase Method[J]. FOOD SCIENCE, 2011, 32(6 ): 237-241.

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Determination of SOD Specific Activity in Animal and Plant Tissues by Improved Xanthine Oxidase Method

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