Analysis of Isoflavonoids in Soybean by Rapid Resolution Liquid Chromatography-Quatrupole-Time of  Flight Mass Spectrometry

doi:10.7506/spkx1002-6630-201106054

FOOD SCIENCE ›› 2011, Vol. 32 ›› Issue (6 ): 233-236.doi: 10.7506/spkx1002-6630-201106054

• Analysis & Detection • Previous Articles Next Articles

Analysis of Isoflavonoids in Soybean by Rapid Resolution Liquid Chromatography-Quatrupole-Time of Flight Mass Spectrometry

ZHENG Zhen-jia1,2，CHU Yu-sheng3，CHI Bing-hai3，WANG Xiao1,2,*

1. College of Food Science and Engineering, Shandong Agricultural University, Taian 271018, China；
2. Shandong Analysis and Test Center, Shandong Academy of Sciences, Jinan 250014, China；
3. Shandong Longda Foodstuff Group Co. Ltd., Yantai 265231, China

Received:2010-04-30 Revised:2011-02-10 Online:2011-03-25 Published:2011-03-03
Contact: WANG Xiao E-mail:wxjn1998@126.com

Abstract

Abstract: A novel method for the analysis of isoflavonoids in soybean was established by rapid resolution liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry (RRLC-Q-TOF-MS). Samples were extracted with methanol under ultrasound assistance, and then a Welch Materials C18 column was applied in the RRLC separation using acetonitrile and water (0.1% formic acid) as the mobile phase. Elutes were detected by Q-TOF to obtain MS spectra with a positive ion mode. Nine isoflavonoids were identified. Therefore, this method can be used to rapidly determine isoflavonoids of soybean.

Key words: RRLC-Q-TOF, soybean, isoflavonoids

CLC Number:

O657.63

ZHENG Zhen-jia1,2，CHU Yu-sheng3，CHI Bing-hai3，WANG Xiao1,2,*. Analysis of Isoflavonoids in Soybean by Rapid Resolution Liquid Chromatography-Quatrupole-Time of Flight Mass Spectrometry[J]. FOOD SCIENCE, 2011, 32(6 ): 233-236.

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Analysis of Isoflavonoids in Soybean by Rapid Resolution Liquid Chromatography-Quatrupole-Time of Flight Mass Spectrometry

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