FOOD SCIENCE ›› 2011, Vol. 32 ›› Issue (8): 88-92.doi: 10.7506/spkx1002-6630-201108020
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CAO Xiang-yu,LIU Jian-li*,LU Xiu-li,LI Qi-jiu,HOU Xiao
Online:
Published:
Abstract: Two extraction methods, namely alkaline method and salt method were used to extract protein from Job s Tears seeds, and their effects on the extraction rate of protein from Job s Tears seed were compared. In the alkaline extraction method, the extraction parameters pH, material-to-liquid ratio, temperature and extraction time were optimized by single factor and orthogonal array design methods based on the extraction rate of protein from Job s Tears seed. The extraction parameters optimized (also by single factor and orthogonal array design methods) in the salt extraction method were salt concentration, material-to-liquid ratio, temperature and extraction time. The results showed that the optimum material-to-liquid ratio, temperature, extraction time and pH for the alkaline extraction of Job s Tears seed protein were 1:12, 35 ℃, 5 h and 11, respectively. Under these conditions, the extraction rate of protein was up to 43.56%. Overall, the alkaline extraction method was superior to the salt extraction method.
Key words: Job s Tears seed, protein, extraction
CLC Number:
TQ93
CAO Xiang-yu,LIU Jian-li,LU Xiu-li,LI Qi-jiu,HOU Xiao. Comparative Study on Methods for Extracting Protein from Job’s Tears Seed[J]. FOOD SCIENCE, 2011, 32(8): 88-92.
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URL: https://www.spkx.net.cn/EN/10.7506/spkx1002-6630-201108020
https://www.spkx.net.cn/EN/Y2011/V32/I8/88