FOOD SCIENCE ›› 2011, Vol. 32 ›› Issue (10): 124-129.doi: 10.7506/spkx1002-6630-201110028

• Analysis & Detection • Previous Articles     Next Articles

Determination of Sulfadianzine Residues in Pork by Direct Competitive Chemiluminescence Enzyme Immunoassay

CHU Jin-shen,XU Yang*,HE Qing-hua,WANG Liu-hua,LU Jiang   

  1. State Key Laboratory of Food Science and Technology, Sino-Germany Joint Research Institute, Nanchang University, Nanchang 330047, China
  • Online:2011-05-25 Published:2011-04-08

Abstract: A direct competitive chemiluminescence enzyme immunoassay (DC-CLEIA) was developed to determine sulfadiazine (SD) residues in pork. Assay parameters including coating antigen (OVA-SD) and enzyme labeled antibody concentrations concentrations and reaction time were optimized to be 0.8μg/mL, 2000-fold dilution and 25 min, respectively. The analytical sensitivity of the developed method was 2.96 ng/mL, and the intra-assay and inter-assay coefficients of variation were below 8.32% and 13.4%, respectively. The recoveries for SD in negative pork spiked at three levels ranged from 75.6% to 103.7%. The McAb had no noticeable cross-reactivity to SD analogues. The standard curve displayed good linearity over the range of 4.14 to 244 ng/mL with a correlation coefficient of 0.9922. This DC-CLEIA may be used for routine detection and monitoring of SD in pork.

Key words: sulfadiazine, direct competitive chemiluminescence enzyme immunoassay, horseradish peroxidase, pork

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