Determination of Lignins in Schisandra sphenanthera and Schisandra chinensis by High Performance Liquid Chromatography

doi:10.7506/spkx1002-6630-201110051

Abstract

Abstract: Objective: To establish a HPLC method for the determination of schisandrin, schisandrol B, deoxyschizandrin and schisandrin B in Schisandra sphenanthera Rehd.et Wils. and Schisandra chinensis (Turcz.) Baill. from 17 growth areas. Methods: The chromatographic separation was carried out on Diamonsil C18 (250 mm× 4.6 mm, 5 μm) column using methanol-water (73:27, V/V) as the mobile phase at a flow rate of 1 mL/min. The detection wavelength was 250 nm. Results: A good linear relationship was achieved with a correlation coefficient in the range of 0.01 to 0.08 mg/mL (R2 = 0.9990) and a RSD of 0.86%, 0.37%, 0.19% and 0.15%, respectively. Conclusion: The developed HPLC method reveals a good separation efficiency, reproducibility and accuracy. This method can be used for determining schisandrin, schisandrol B, deoxyschizandrin and schisandrin B in herbs. Higher amounts of schisandrin, schisandrol B and schisandrin B are present in Schisandra chinensis than in Schisandra sphenanthera.

Key words: Schisandra sphenanthera Rehd.et Wils., Schisandra chinensis (Turcz.) Baill., high performance liquid chromatography (HPLC), lignin

CLC Number:

R284.1

YIN Guan-xiu，DU Bing，LU Wang-wang，HUA Yang-lin，TANG Jian，YANG Gong-ming. Determination of Lignins in Schisandra sphenanthera and Schisandra chinensis by High Performance Liquid Chromatography[J]. FOOD SCIENCE, 2011, 32(10): 218-221.

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Determination of Lignins in Schisandra sphenanthera and Schisandra chinensis by High Performance Liquid Chromatography

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