Physiological, Biochemical and Molecular Biological Identification of a Xylitol-producing Strain

doi:10.7506/spkx1002-6630-201111037

Abstract

Abstract: In this paper, an unreported strain (CTD249) with the capability of transforming xylose to xylitol was identified by physiological, biochemical and molecular biological methods. Meanwhile, a phylogenetic tree was constructed to analyze the genus which the strain CTD249 belongs to. The results indicated that the strain CTD249 and Pichia guilliermondii were in the same branch with more than 99% sequence similarity. This strain was identified as Pichia guilliermondii. With the initial xylose concentration of 20 g/L, the output of xylitol was up to 12.0 g/L after 48 h cultivation.

Key words: xylitol, Pichia guilliermondii, phylogenetic analysis, identification

CLC Number:

Q532.2

QU Jia-le，MIN Wei-hong，WANG Qiao-ling，FANG Li. Physiological, Biochemical and Molecular Biological Identification of a Xylitol-producing Strain[J]. FOOD SCIENCE, 2011, 32(11): 171-175.

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Physiological, Biochemical and Molecular Biological Identification of a Xylitol-producing Strain

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