Ultrasonic-assisted Extraction of Total Flavonoids from Gynura divaricata L. DC

doi:10.7506/spkx1002-6630-201114029

FOOD SCIENCE ›› 2011, Vol. 32 ›› Issue (14): 144-146.doi: 10.7506/spkx1002-6630-201114029

• Processing Technology • Previous Articles Next Articles

Ultrasonic-assisted Extraction of Total Flavonoids from Gynura divaricata L. DC

LI Hui1,2，BU Xiao-ying3，CHEN Gong-xi1,2，LI Ya-nan1，LIANG Chun-feng1，XIE Yi-zhen1

(1. College of Biology Resources and Environmental Science, Jishou University, Jishou 416000, China； 2. Key Laboratory of Plant Resource Conservation and Utilization of College of Hunan Province, Jishou 416000, China ； 3. Key Laboratory of Forstry Product and Chemical Engineering of Hunan Province, Zhangjiajie 427000, China)

Online:2011-07-25 Published:2011-06-18

Abstract

Abstract: Objective: To optimize the ultrasonic-assisted extraction (UAE) of total flavonoids from dried and fresh leaves and stems of Gynura divaricata L. DC and explore the mechanisms by which ultrasonic can enhance the extraction of total flavonoids. Methods: The effects of material-to-liquid ratio, extraction time and ethanol concentration on extraction rate of total flavonoids were explored by single factor experiments. Scanning electron microscope (SEM) was utilized for examining the structural change of Gynura divaricata L. DC leaves after ultrasonic treatment. Results: The optimal UAE conditions were material-to-liquid ratio of 1: 30 (g/mL); extraction time of 15 min, ethanol concentration of 70%, and number of repeated extractions of 2. Under these conditions, the extraction rate of total flavonoids from the same parts of fresh Gynura divaricata L. DC was higher than that from dried Gynura divaricata L. DC. Conclusion: UAE method has many advantages over conventional solvent extraction such as higher extraction efficiency and shorter extraction time, which is possibly due to severe damage of Gynura divaricata L. DC cell wall by ultrasound.

Key words: ultrasonic-assisted extraction (UAE), Gynura divaricata L. DC, total flavonoids

CLC Number:

LI Hui，BU Xiao-ying，CHEN Gong-xi，LI Ya-nan，LIANG Chun-feng，XIE Yi-zhen. Ultrasonic-assisted Extraction of Total Flavonoids from Gynura divaricata L. DC[J]. FOOD SCIENCE, 2011, 32(14): 144-146.

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Ultrasonic-assisted Extraction of Total Flavonoids from Gynura divaricata L. DC

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