Abstract: An ion-pair reversed-phase high performance liquid chromatographic method was developed to simultaneous determine fructose, glucose, and maltose in honey using p-aminobenzoic acid (p-AMBA) for pre-column derivatization. The chromatographic separation was achieved on an ODS column using a mobile phase made up of 0.1 mol/L phosphate buffer solution (20 mmol/L tetrabutylammoniumhydrogen sulfate, pH 2.5) and acetonitrile (95:5, V/V). The analytes were detected using a fluorescence detector and quantified by external standard method. The results showed that the detection limits of fructose, glucose and maltose were 210, 96μg/kg and 240μg/kg, respectively, and the linear ranges were 130 －140, 52－98 mg/kg, and 154－98 mg/kg (r ＞ 0.9988). The spike recoveries ranged from 98.2% to 101.2%， with RSDs between 3.4% and 5.4%.

Key words: high performance liquid chromatographic, fluorescence detection, honey, reducing sugars