FOOD SCIENCE ›› 2011, Vol. 32 ›› Issue (16): 187-191.doi: 10.7506/spkx1002-6630-201116039

• Processing Technology • Previous Articles     Next Articles

Separation of Antioxidant Peptides from Maize by Macroporous Resin Adsorption

DIAO Jing-jing1,CAO Long-kui1,2,*   

  1. (1. Agri-food Processing Development Centre of Heilongjiang, Daqing 163319, China; 2. College of Food Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 163319, China)
  • Online:2011-08-25 Published:2011-07-26

Abstract: In this study, three types of macroporous resin were assessed for their adsorption and desorption capacity towards antioxidant peptides in the maize protein hydrolysate, prepared by alcalase hydrolysis, and XAD-7HP resin was found to be the most suitable resin among them. The static adsorption of XAD-7HP resin and the static desportion of antioxidant peptides with different desorption solvents were performed under different pH conditions. As a result, the optimal process parameters for the separation of antioxidant peptides were sample pH 7.0 and 70% aqueous ethanol as the desorption solvent. Antioxidant maize peptides were successfully separated by the dynamic adsorption of XAD-7HP resin, resulting in a 2-fold increase in DPPH free radical scavenging activity.

Key words: macroporous resin, antioxidant maize peptides, static adsorption, desorption capacity

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