Isolation and Identification of Sporisorium reilianum

doi:10.7506/spkx1002-6630-201117050

Abstract

Abstract: Objective: To isolate and identify Sporisorium reilianum from sorghum cultivar Jiliang 105A . Methods: Single spores were isolated from samples. The morphology of isolated spores was examined and the internal transcribed spacer (ITS) was sequenced. Results: The strain grown on the natural solid culture medium revealed leather face as well as jagged and brownish-black edge. The amplified fragment length of ITS was 721 bp. Based on sequence alignment carried out using DNAStar software, the isolated strain was identified as Sporisorium reilianum. Conclusion: Sporisorium reilianum is successfully isolated and identified from Jiliang 105A .

Key words: Sporisorium reilianum, internal transcribed spacer (ITS), isolation, identification

CLC Number:

TS201. 3

LU Xiu-li，YAO Yan-fei，WANG Xin. Isolation and Identification of Sporisorium reilianum[J]. FOOD SCIENCE, 2011, 32(17): 243-245.

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Isolation and Identification of Sporisorium reilianum

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