Abstract: Objective: To establish a method for determining naringin, hesperidin, and neohesperindin in Citrus paradisi cv. Changshan Huyou fruit and compare their contents in different parts such as peel, pulp and seeds. Methods: The contents of naringin, hesperidin and neohesperindin were determined by a reversed-phase HPLC method. Methanol extracts of samples and the standard substances were separated on a YMC C18 column (4.6 mm × 250 mm, 5μm) using a mobile phase made up of acetonitrile and water (23:77 , V/V) at a flow rate of 1 mL/min and detected by a UV detector at a wavelength of 283 nm. The column temperature was set at 25 ℃. Results: The calibration curves were linear in the range of 3.22－1030μg/mL for naringin, 0.59－380μg/mL for hesperidin, and 0.077－990μg/m for neohesperindin, respectively. The average recoveries of naringin, hesperidin and neohesperindin were 98.43% (RSD = 4.07%), 98.70% (RSD = 4.30%) and 99.90% (RSD = 3.47%), respectively. The contents of naringin, hesperidin, neohesperindin were the highest in the peel and the lowest in the seeds of Citrus paradisi cv. Changshan Huyou fruit. Conclusions: This method is simple, accurate, reliable and reproducible and can therefore be used for the quality control of Citrus paradisi cv. Changshan Huyou fruit.

Key words: Citrus paradisi cv. Changshan Huyou, naringin, hesperidin, neohesperindin, determination