FOOD SCIENCE ›› 2011, Vol. 32 ›› Issue (18): 214-217.doi: 10.7506/spkx1002-6630-201118046

• Analysis & Detection • Previous Articles     Next Articles

Simultaneous Determination of Eight Catechins, Three Purine Alkaloids and Gallic Acid in Tea by High-performance Liquid Chromatography

LI Yin-hua,LI Juan,GONG Xue,LIU Zhong-hua*   

  1. (National Research Center of Engineering Technology for Utilization of Functional Ingredients from Botanicals, Key Laboratory of Tea Science, Ministry of Education, Hunan Agricultural University, Changsha 410128, China)
  • Online:2011-09-25 Published:2011-09-24

Abstract: A method was developed to determine catechins, purine alkaloids and gallic acid in tea by HPLC-PDA. The optimum condition was achieved by separation on a Welchrom C18 column kept at 30 ℃ using phosphate buffer-acetonitrile as the mobile phase at a flow rate of 1.0 mL/min through gradient elution and detection at 278 nm. This method showed a good linearity for all 12 tested compounds. The results demonstrated high accuracy and stability of this method.

Key words: high performance liquid chromatography (HPLC), catechin, methylated EGCG, purine alkaloid, gallic acid

CLC Number: