FOOD SCIENCE ›› 2011, Vol. 32 ›› Issue (18): 264-267.doi: 10.7506/spkx1002-6630-201118059

• Analysis & Detection • Previous Articles     Next Articles

A Method for Screening and Detection of Virulence Genes of Vibrio Isolates from Freshwater Crayfish

LI Xiao-jing1,ZHAO Hui2,SU Jing1,GUO Ai-ling1,*,ZHANG Yu1   

  1. (1. College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430000, China; 2. Hubei Entry-Exit Inspection and Quarantine Bureau, Wuhan 430000, China)
  • Received:2018-06-20 Revised:2018-06-20 Online:2011-09-25 Published:2011-09-24

Abstract: A specific and sensitive PCR method for the detection of Vibrio was established. The ctxA, zot and RS1 genes of CTX genetic element, tcpA and toxT genes of VPI pathogenic island, rtxC gene of RTX cluster, and toxR, T139, tcpH, toxRS, toxS and ctxAB genes in 6 Vibrio isolates reported in the literature were used to design specific primers for PCR. The results indicated that the established method was simple, easy, specific and time-saving. As a result, it can effectively and efficiently detect virulence genes of Vibrio strains.

Key words: Vibrio, polymerase chain reaction (PCR), virulence gene, identification

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