FOOD SCIENCE ›› 2011, Vol. 32 ›› Issue (19): 75-78.doi: 10.7506/spkx1002-6630-201119016

• Basic Research • Previous Articles     Next Articles

Separation, Purification and Identification of Antibacterial Components in Rape Bee Pollen

SUN Li-ping1,DU Xia1,CHEN You-gen2,*,XU Xiang1,AN Zhong-yao3,LI Chun-yan3   

  1. (1. Bee Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100093, China; 2. Beijing Institute for Drug Control, Beijing 100035, China; 3. College of Resources and Environmental Sciences, Northeast Agricultural University, Harbin 150030, China)
  • Published:2011-10-12

Abstract: The bioassay-guided fractionation was used to isolate antibacterial components from aqueous extract of rape bee pollen. The MICs and MBCs of antibacterial components isolated against Bacillus subtilis, Escherichia coli, Staphylococcus aureus rosenbach and Pseudomonas aeruginosa were determined. An active component with strong antibacterial activity was obtained and identified as kaempferol by ESI-MS and NMR spectroscopy. Therefore, rape bee pollen is a natural antibacterial plant source and has promising application potential.

Key words: rape bee pollen, antibacterial component, structural identification

CLC Number: