FOOD SCIENCE ›› 2019, Vol. 40 ›› Issue (22): 299-305.doi: 10.7506/spkx1002-6630-20181128-341

• Processing Technology • Previous Articles     Next Articles

New Yellow Pigment from Cordyceps militaris: Structural Identification and Optimization of Extraction by Response Surface Methodology

TANG Hongbiao, CHEN Chuxin, LIN Junfang,, LOU Haiwei, YE Zhiwei, GUO Liqiong,   

  1. (1. College of Food Science, South China Agricultural University, Guangzhou 510642, China; 2. Research Center for Micro-Ecological Agent Engineering and Technology of Guangdong Province, Guangzhou 510642, China)
  • Online:2019-11-25 Published:2019-12-02

Abstract: This study was done in order to investigate the yellow pigment composition of Cordyceps militaris and to determine the optimal processing conditions for the extraction of?yellow pigment from C. militaris. We applied high performance liquid chromatography (HPLC), high-resolution mass spectrometry (HRMS) and Fourier transform infrared (FTIR) spectroscopy to the separation, purification and structural identification of yellow pigment. A new yellow pigment was found and named cordycepene, which had similar UV-visible and infrared absorption characteristics to carotenoids. By using the response surface methodology, the optimal extraction conditions were obtained as follows: 57.68% ethanol as the extraction solvent and extraction for 52.44 min at 44.40 ℃. Under these optimized conditions, the highest yield of cordycepene of (2 780.97 ± 170.38) μg/g was obtained.

Key words: Cordyceps militaris, yellow pigment, structural identification, cordycepene, extraction optimization, response surface methodology

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