Abstract: The sequence of 2031 site in anterior HBV C/C area was selected as the substrate RNA fragment to synthesize the corresponding 10-23 DNAzyme. Under mimic physiological conditions, the thermodynamics of 10-23 DNAzyme was evaluated by microcalorimic method. Heat-flow curves were plotted to obtain the initial reaction rate (v0) at various concentrations of the substrate RNA. The maximum rate (vmax), Michaelis-menten constant (Km) and catalytic constant (Kcat) of 10-23 DNAzyme were obtained from the established Lineweaver-Burk plot of 1/v0 against 1/[S] to be 2.5 × 10-9 mol/(L ·s), 0.045 nmol/L and 0.107 min-1, respectively.

Key words: 10-23 DNAzyme, cleavage reactio, microcalorimetric method, thermodynamics