Separation and Purification of Black Soybean Peptide and Its Hypolipidemic Effect in Mice

doi:10.7506/spkx1002-6630-201119056

Abstract

Abstract: Macroporous adsorption resin DA201-C was used for the isolation of hypolipidemic peptides from black soybean protein hydrolysate. The inhibitory rates of cholesterol micelle solubility (CMSIR) by all fractions were determined, and the hypolipidemic effect of the fraction with the highest CMSIR was evaluated in mice. The results showed that black soybean peptides were eluted from DA201-C resin according to its hydrophobicity through gradient ethanol elution. The peptide fraction eluted by 75% ethanol displayed the highest CMSIR of 62.1% when the concentration of peptide fraction was 5 g/L. After administration of the peptide at the dose of 1000 mg/(kg ·d) for 30 days in mice, the levels of serum TC, TG and LDL-C and VLDL-C were obviously decreased (P <0.01) when compared with the mice fed hypercholesterolemia diet.

Key words: black soybean peptides, macroporous resin, separation and purification, cholesterol micelle solubility, hypolipidemic activity

CLC Number:

TS201.2

LIU En-qi，WU Yong-hua， ZHANG Jian-ping， CHEN Zhen-jia， LI Yu-e. Separation and Purification of Black Soybean Peptide and Its Hypolipidemic Effect in Mice[J]. FOOD SCIENCE, 2011, 32(19): 248-252.

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Separation and Purification of Black Soybean Peptide and Its Hypolipidemic Effect in Mice

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