Isolation, Purification and Monosaccharide Composition Analysis of Polysaccharides from Two Umbilicaria Species

doi:10.7506/spkx1002-6630-201121011

Abstract

Abstract: Objective：To analyze the monosaccharide composition of polysaccharides extracted from two Umbilicaria species: Umbilicaria decussate (Vill.) Zahlbr and Umbilicaria virginis Schaer.. Methods: Water-soluble polysaccharides from both Umbilicaria species were obtained by water extraction, ethanol precipitation and freeze-dried and purified into fractions SUv and ZUd, respectively by sequential column chromatography on DEAE-52 and Sephadex G-150. Gas chromatography (GC) was used to analyze their monosaccharide composition. Results: The monosaccharide composition of crude Umbilicaria virginis polysaccharides was composed of mannose, glucose and galactose at a ratio of 16.00:50.96:6.17. The monosaccharide composition of SUv was comprised of mannose, glucose and galactose at a ratio of 2.97:17.70:1.52. The monosaccharide composition of crude Umbilicaria decussate polysaccharides was made up of mannose, glucose and galactose at a ratio of 3.90:38.086:2.00. ZUd contained only 5.37% glucose. Conclusion: Both Umbilicaria decussata and Umbilicaria virginis contain glucose and a small amount of mannose and galactose.

Key words: Umbilicaria decussate (Vill.) Zahlbr., Umbilicaria virginis Schaer., gas chromatography, polysaccharides, monosaccharides

CLC Number:

TS201.4

Gulzar ABDUKEYUM，Nurbiya YALKUN，Kerimjan TURSUNJAN，Abdulla ABBAS. Isolation, Purification and Monosaccharide Composition Analysis of Polysaccharides from Two Umbilicaria Species[J]. FOOD SCIENCE, 2011, 32(21): 48-51.

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Isolation, Purification and Monosaccharide Composition Analysis of Polysaccharides from Two Umbilicaria Species

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