Screening and Identification of High Protease-producing Bacteria

doi:10.7506/spkx1002-6630-201121037

FOOD SCIENCE ›› 2011, Vol. 32 ›› Issue (21): 183-187.doi: 10.7506/spkx1002-6630-201121037

• Bioengineering • Previous Articles Next Articles

Screening and Identification of High Protease-producing Bacteria

MA Gui-zhen，BAO Zeng-hai，WANG Shu-fang，WU Shao-jie，FU Hong-run，GE Ping-hua

(School of Marine Science and Technology, Huaihai Institute of Technology, Lianyungang 222005, China)

Received:2018-06-20 Revised:2018-06-20 Online:2011-11-15 Published:2011-11-11

Abstract

Abstract: Totally 44 bacterial strains were isolated from 9 soil samples collected from Guangdong, Hebei, Liaoning, Hunan and Jiangsu by diluted plate method. The protease-producing ability of the bacteria isolated was screened by transparent cycle plate assay. The results indicated that of these bacterial isolates, 16 exhibited clear transparent cycles. Meanwhile, strains G1-b, GD-2-2 and HX-B-1 revealed the strongest protease activity. The ratio of transparent circle diameter to colony diameter was higher than 3.5. Strain GD-2-2 had stronger protease activity than the other two, reaching 447.6 U/mL. Based on morphological observations and physiological and biochemical tests, strain GD-2-2 belonged to Bacillus. The 16S rDNA sequence analysis showed that the isolate was 100% similar with Bacillus licheniformis strain YB915 (GQ996726.1). Therefore, strain GD-2-2 belonged to Bacillus licheniformis.

Key words: bacteria, protease, identification

CLC Number:

Q939.97

MA Gui-zhen，BAO Zeng-hai，WANG Shu-fang，WU Shao-jie，FU Hong-run，GE Ping-hua. Screening and Identification of High Protease-producing Bacteria[J]. FOOD SCIENCE, 2011, 32(21): 183-187.

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Screening and Identification of High Protease-producing Bacteria

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