Isolation and Identification of a High Exopolysaccharide-producing Strain from Biological Soil Crusts on Sand Dunes

doi:10.7506/spkx1002-6630-201121042

Abstract

Abstract: Twenty-seven stains producing exopolysaccharides were isolated from biological soil crusts in the Gurbantunggut Desert, Xinjiang by the traditional method. A strain with 7445.80 mg/L exopolysaccharide-producing activity was picked out of them by phenol-sulfuric acid method and named as XJ-27. Based on phenotypic and physiological-chemical characterization, XJ-27 was primarily identified as Bacillus thuringiensis and further confirmed through 16S rDNA sequence analysis, resulting in a 1452 bp PCR amplification sequence, homology comparison with NCBI databases using the software Blast and phylogenetic tree construction using the software MEGA 4.0.

Key words: biological soil crusts, exopolysaccharides, identification, Bacillus thuringiensis

CLC Number:

TS201.3

WANG Zheng-rong，SHENG Ji-ping，TIAN Xiao-lei，WU Ting-ting，LIU Wan-zhen，CHENG Fan-sheng，JIAO Yu-cui，SHEN Lin. Isolation and Identification of a High Exopolysaccharide-producing Strain from Biological Soil Crusts on Sand Dunes[J]. FOOD SCIENCE, 2011, 32(21): 207-209.

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Isolation and Identification of a High Exopolysaccharide-producing Strain from Biological Soil Crusts on Sand Dunes

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