FOOD SCIENCE ›› 2011, Vol. 32 ›› Issue (22): 32-36.doi: 10.7506/spkx1002-6630-201122007

• Processing Technology • Previous Articles     Next Articles

Optimization of Microwave Extraction of Flavonoids from Robinia pseudoacacia L. Flowers Using Response Surface Methodology

XIANG Chang-guo1,XIANG Ning2,HUANG Cheng-long3,SUN Hai-bin3,LI Wen-fang3   

  1. (1. Hunan Application Technology of Ecotourism Key Laboratory, Jishou University, Zhangjiajie 427000, China; 2. College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China; 3. College of Resources and Planning Sciences, Jishou University, Zhangjiajie 427000, China)
  • Online:2011-11-25 Published:2011-11-11

Abstract: The optimum conditions for the extraction of flavonoids from Robinia pseudoacacia L. flowers were determined using response surface methodology. On the basis of one-factor-at-a-time experiments, Box-Benhnken central composite design was applied to investigate the effects of three variables, namely alcohol concentration, liquid-to-solid ratio and microwave heating time on extraction yield of flavonoids. A regression model describing extraction yield of flavonoids as a function of these three variables was established. The best results of the extraction of flavonoids from Robinia pseudoacacia L. flowers were obtained when the samples were extracted twice by means of microwave heating for 145 s at a ratio of liquid to solid of 37:1 using 75% ethanol. Under these conditions, the extraction yield of flavonoids was 8.63 mg/g.

Key words: Robinia pseudoacacia L. flowers, flavonoids, response surface methodology, microwave-assisted extraction

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