Abstract: Macroporous resin adsorption was used to purify flavonoids and triterpenoids from crude extract resulting from sequential organic solvent extraction of the whole plant of Kalimeris indica (L.) Sch-Bip. with 80% ethanol, petroleum ether (decolorization) and ethyl acetate and their lipid peroxidation inhibitory activity in vitro was measured. AB-8 was selected as the best resin out of 15 ones by comparing their static adsorption and desorption, adsorption kinetic characteristics and isothermal adsorption curves. Kinetic adsorption and desorption on AB-8 column were used to purify flavonoids and triterpenoids. The results of elution with different concentrations of ethanol showed that 20% ethanol eluate contained the largest amount of flavonoids, reaching 62.7%, and 19% triterpenoids with a total yield of 51.5% and that 60% ethanol eluate had the highest triterpenoid content, reaching 58.5% with a total yield of 22.3%. Both constituents had some inhibitory effect on lipid peroxidation in mouse heart, liver, kidney and brain tissues in vitro, especially on the liver.

Key words: macro-porous resin, Kalimeris indica (L.) Sch-Bip, triterpenoids, flavonoids, lipid peroxidation