Determination of Aleuritic Acid by High Performance Liquid Chromatography with Differential Refractive Index Detector

doi:10.7506/spkx1002-6630-201122055

Abstract

Abstract: In this paper, we present a high performance liquid chromatography (HPLC) with differential refractive index detector (RID) method to determine aleuritic acid. Ten mL of the analyte was chromatographically separated on an Agilent ZORBAX SB-C18 column (4.6 mm × 250 mm, 5μm) using a mobile phase consisting of a mixture of methanol and water (60:40, containing 0.1% trifluoroacetic acid) at a flow rate of 1 mL/min. The column temperature was set as 30 ℃. Aleuritic acid showed a good linear relationship over the concentration range of 0.01-1.0 mg/mL with a correlation coefficient of 0.9994. The limit of detection was 0.008 mg/mL. The precision RSD was 0.86% (n ＝9). The average spike recovery rate was 100.23% with a RSD of 0.65% (n ＝ 9). The method was easy to operate, simple, accurate, stable and reliable.

Key words: aleuritic acid, high performance liquid chromatography (HPLC), differential refractive index detector (RID)

CLC Number:

TS207.3

LIUShi-ping，ZHANGHong，ZHOUMei-cun，ZHENGHua，LIKai，GUOYuan-heng. Determination of Aleuritic Acid by High Performance Liquid Chromatography with Differential Refractive Index Detector[J]. FOOD SCIENCE, 2011, 32(22): 260-263.

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Determination of Aleuritic Acid by High Performance Liquid Chromatography with Differential Refractive Index Detector

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